人基质金属蛋白酶抑制因子2(TIMP2)ELISA试剂盒常见问题解答：

1.问：我的标准曲线看起来很好，但我没有在样本中得到预期的信号，这是为什么？答：样品可能不含分析物。矩阵效应可能掩盖检测。确保按照我司试剂盒中说明书进行稀释。稀释，前请检查以确保稀释操作正确。过度稀释可能导致样品低于标准曲线的范围。Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

2.问：你建议我该如何洗板？答：如果您使用的是自动洗板机，我们建议定期检查校正，并在清洗前用洗板机缓冲液冲洗系统。手动洗板机也是如此。或者使用中继器或洗涤瓶。用户应小心确保所有内容物都被吸入，并在无棉纸上轻敲干燥。Q:How do you recommend I wash my plate?A:If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper.人基质金属蛋白酶抑制因子2(TIMP2)ELISA试剂盒使用注意事项：1. 本试剂盒取出的试剂在室温条件下使用，溶液应轻轻摇匀后使用。从冰箱中取出的浓缩洗涤液可能有结晶，这属于正常现象，加热使结晶完全溶解后再配制。2. 底物液使用前建议先行检查，若溶液颜色发蓝则已失效，弃去。3. 以上标准孔及待查样品均建议做复孔，每次测定应同时制作标准曲线。4. 本试剂盒含 2M 硫酸，不要将它与含叠氮化钠的废物混在一起。5. 板条开封后剩余板条要密封好，保持板条干燥。6. 不同批号的试剂盒组分不能混用。7.正确拿酶标板：不要接触板的底部，否则会影响度数。