犬肝素辅因子Ⅱ(HCⅡ)elisa试剂盒的注意事项:1) 充分混匀对保证反应结果很重要,在加液后请轻轻晃动整个96板孔,以保证混匀。2) TMB溶液请勿接触氧化剂或金属,否则容易失效。3) 加样时请注意每个样品或标准品必须更换枪头,一方面避免交叉污染,另一方面也避免吸取体积的误差。加样过程中必须避免气泡的产生。4) 检测标准品和样品时建议设置重复孔,以确保检测结果的可信度。5) 洗涤过程非常重要,洗涤不充分会下降并导致结果误差较大。
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Calculation of results 1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software