猪三碘甲腺原氨酸(T3)ELISA试剂盒常见问题解答:
1. 问:我期望得到的分析物的预期浓度是多少?
答:给定分析物的量不仅可能因物种而异,也可能因组织和细胞来源而异。这些信息的**来源是通过互联网在多个科学数据库中轻松访问的文献。
Q:What is the expected concentration of analyte that I should expect to find?
A:The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.
2. 问:我的光密度比试剂盒中附带的说明书里的光密度稍高(或更低),这是为什么?
答:光密度受很多物理条件的影响,如时间和温度。我们建议您缩短或延长基质溶液的*终孵育时间以进行补偿。
Q:My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?
A:The optical density is affected by a number of physical conditions such as time and temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.
上海轩轩泽康生物猪三碘甲腺原氨酸(T3)ELISA试剂盒产品参数:
试剂盒成份(2-8℃保存) | 英文名称 | 96孔配置/48孔配置 | 配制 |
微孔酶标板 | Microelisa stripplate | 12孔×8条/12孔×4条 | 即用型 |
标准品 | Standard | 0.3ml*6管/0.3ml*6管 | |
生物素标记抗体 | Biotinylated antibody | 6ml / 3ml | |
样本稀释液 | Sample Diluent | ||
检测抗体-HRP | HRP-Conjugate reagant | 10ml / 5ml | |
20×洗涤缓冲液 | 20× Wash solution | 25ml / 15ml | 按说明书进行稀释 |
底物A | Chromogen Solution A | ||
底物B | Chromogen Solution B | ||
终止液 | Stop Solution | ||
封板膜 | Closure plate membrane | 2张/2张 | |
说明书 | User manual | 1份/1份 | |
自封袋 | Sealed bags | 1个/1个 | |
特别说明: 1)(96T/48T)打开包装后请及时检查所有物品是否齐全。 2)20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份20×洗涤缓冲液加19份蒸馏水。 3)一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃。 |