人甲胎蛋白异质体3(αFPL3)ELISA试剂盒注意事项:
1.在试验中标准品和样本检测时建议做双孔检测,每次检测都应做标准曲线。
2.洗涤过程很关键,洗涤不充分将导致精,确度误差及OD值错误的升高,从冰箱中取出的浓缩洗涤液可能有结晶属于正常现象。37℃水浴结晶完,全溶解后再配置洗涤液。
3.检测时所有试剂都要恢复到室温,板条开封后剩余板条需封存,放回袋中1个月内用完。
4.试剂盒使用超敏TMB溶液,显色过深时会出现沉淀状,属于正常现象,混匀即可。不影响结果判读。
5.说明书以试剂盒内纸质版为准。本试剂盒仅用于科研,不能用于临床诊断!
人甲胎蛋白异质体3(αFPL3)ELISA试剂盒常见问题解答:
1.问:我可以改变化验中使用的样本体积吗?答:不建议更改容量,因为我司所有试剂盒的组分套件都是为在给容量下实现zui佳性能而设计的。Q:Can I alter the volume of sample I use in the assay?A:It is not recommended that you alter the volumes since all kits are designed for optimal performance at the given volumes
2.问:是否可以使用不同试剂盒的组分?答:每一个试剂盒包含具有特定批号的组件,以确保所有组件单独以及试剂盒中所有其他组件都能达到zui佳性能。对这些特定批次进行质量控制测试。绝不建议用户使用自己的组件或其他供应商试剂盒。Q:Can components from different kits be used?A:Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.