| 货号: 26921 |
| 规格: 30 µL |
| 基因符号: RAB4 |
| 描述: Anti-Rab4-GTP Monoclonal Antibody |
| 背景介绍: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, M1, M2 and M3. It participates in protein transport and in vesicular traffic. |
| 免疫原: Recombinant full length protein of active Rab4A. |
| 经过测试的应用: IP, IHC, IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: Rab4A Activation Assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |
Rab4A Activation Assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.