| 货号: 26913 |
| 规格: 30 µL |
| 基因符号: Ral |
| 描述: Anti-Ral-GTP Mouse Monoclonal Antibody |
| 背景介绍: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation. |
| 免疫原: Recombinant full length protein of active RalB. |
| 经过测试的应用: IP, IHC, IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: no |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹:  Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Ral-GTP monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033). |