黑曲霉
平台编号：bio-67649 国际编号：ATCC 16404 拉丁属名： Aspergillus brasiliensis 规格：freeze-dried 用途：ATCC原装进口
Aspergillus brasiliensis Varga et al.拉丁名
(ATCC? 16404?) 统一编号
Deposited As Aspergillus niger van Tieghem
Strain Designations 菌种别名 WLRI 034(120) [CBS 733.88, , IFO 9455, IMI 149007, NCPF 2275]
Application 用途 Assay of antimicrobial preservatives； Media testing； Preparatory test control； Quality control strain； Sterility testing； Testing fungicides； Transformation host； Validation control；
Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM) Food testing Pharmaceutical and Personal Care
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with
biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 生长条件
Frozen 冷冻物: -80°C or colder
Freeze-Dried 冻干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? no
Comments 注释 This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified. This strain was recently re-named as Aspergillus brasiliensis. Use of impedance for preservative efficacy testing Morphology Colonies initially white or yellowish, mycelium growing rapidly producing a dense layer of erect smooth-stiped, conidiophores terminated by globose vesicles bearing phialides (uniseriate) or metulae with phialides (biseriate) which produce dry chains of conidia. Reverse pale to grayish or greenish yellow. Vesicles radiate, initially pale, becoming dark brown to black. Conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) μm in diameter. Sporulation may be inhibited when grown in vessels with reduced gas exchange. Colonies may exhibit sectoring with areas of varying levels of sporulation. Use of freshly produced spores as inoculum should reduce sectoring.
Medium 培养基 ATCC? Medium 336: Potato dextrose agar (PDA)
ATCC? Medium 325: Malt extract agar (Blakeslee's formula)
ATCC? Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions 生长条件
Temperature 培养温度: 20°C to 25°C
Atmosphere 需氧情况: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCCAACCTCCCATCCGTGTCTATTGTACCCTGTTGCTTCGGCGGGCCCGCCGCTTGTCGG
CCGCCGG
GGGGGCGCCTCTGCCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACACGAACCCTGTCTGAAAGCGTGCAGTCTGAGTCGATTGTTTGCAATCAGTTAAAACTTTCAACAATGGATCTCT
TGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGG
GGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGCCGTCCCCTCTCTCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCG
ATCCTCGAGCGTATGGGGCTTTGTCACATGCTCTGTAGGATTGGCCGGCGCCTGCCGACGTTTTCCAACCATTCTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAAC
TTAAGCATATCAATAA D1D2 region of the 28S Ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGAGTCCGCATTGTAATTTGCAGA
GGATGCTTTGGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTTGGGCGGGGTGTCCGTGCCCGTGTAAAGCTCCTTCGACGAGTCGAGTTG
TTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGA
GAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGTGGGCGGGCCAGCGTCGGTTTGGGC
GGCCGGTCAAAGGCCCCTGGAATGTAGTGCCCTCCGGGGCACCTTATAGCCAGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAA
CGAC
Morphology Colonies initially white or yellowish, mycelium growing rapidly producing a dense layer of erect smooth-stiped, conidiophores terminated by globose vesicles bearing phialides (uniseriate) or metulae with phialides (biseriate) which produce dry chains of conidia. Reverse pale to grayish or greenish yellow. Vesicles radiate, initially pale, becoming dark brown to black. Conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) μm in diameter. Sporulation may be inhibited when grown in vessels with reduced gas exchange. Colonies may exhibit sectoring with areas of varying levels of sporulation. Use of freshly produced spores as inoculum should reduce sectoring.
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