货号: 26915 |
规格: 30 µL |
基因符号: RAN |
描述: Anti-Ran-GTP Mouse Monoclonal Antibody |
背景介绍: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. |
免疫原: Recombinant full length protein of active Ran. |
经过测试的应用: IP, IHC, IF |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
浓度: 1 mg/ml |
宿主: mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Anti-Ran-GTP monoclonal antibody recognizes active Ran of vertebrates. |
储存条件: Store at -20°C. Avoid freeze/thaw cycles |
免疫沉淀/蛋白质印迹: Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Ran-GTP monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody. |