| 货号: 26910 |
| 规格: 30 µL |
| 基因符号: RHEB |
| 描述: Anti-Rheb-GTP Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR). |
| 免疫原: Recombinant full-length protein of active Rheb. |
| 经过测试的应用: IP, IHC,IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgM |
| 纯度: Purified from ascites |
| 防腐剂: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates. |
| 储存条件: Store at -20°C. Avoid freeze/thaw cycles |
免疫沉淀/蛋白质印迹:  Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098). |